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氯化鈉多粘菌素B肉湯spb配方

發(fā)布時間:

2022-12-27

作者:

日水生物


氯化鈉多粘菌素B肉湯配制方法:
稱取本品33.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱至完全溶解,分裝,121℃高壓滅菌15分鐘,冷卻至55℃,每100ml加入一支20519【多粘菌素 B( 2.5 萬單位)】,搖勻,備用。
 
Formula(氯化鈉多粘菌素B肉湯配方): g/L
 
Peptone(蛋白胨)                                   10.0g
Yeast Extract(酵母浸出粉)                   3.0g
Sodium Chloride(氯化鈉)                    20.0g
pH7.4±0.1(25℃)
 
 
Preparation method:
According to take this product 33.0 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), high heat until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, cool to 55 ℃, 100 per 100 ml join a 【 polymyxin B (25000 units) 】, shake well, set aside.
 
Formula: g/L
 
Peptone (Peptone) 10.0g
Calcium Extract (Yeast Extract) 3.0g
Sodium Chloride (nacl) 20.0 g
PH7.4 slightly + / - 0.1 (25 ℃)