10%氯化鈉胰蛋白胨大豆肉湯制備方法
發(fā)布時間：
2022-12-27
作者：
日水生物
10%氯化鈉胰蛋白胨大豆肉湯配制方法: 稱取本品125.0g于1L蒸餾水或去離子水中可按比例增加或減少配制量），微溫溶解，分裝，121℃高壓滅菌15分鐘,備用。制備雙料胰蛋白胨大豆肉湯時，除蒸餾水外，其他成分加倍。
Formula(10%氯化鈉胰蛋白胨大豆肉湯配方)： g/L
Tryptone胰蛋白胨 17.0g
Enzymatic Digest of Soybean Meal
植物蛋白胨 3.0g
Sodium Chloride(氯化鈉 100.0g
Dipotassium Phosphate
磷酸氫二鉀 2.5g
Dextrose葡萄糖 2.5g
pH7.3±0.2(25℃)
Preparation method: it is called 125.0g in 1L distilled water or deionized water to increase or reduce the amount of preparation according to proportion), micro temperature dissolving, separation, 121 centigrade high pressure sterilization for 15 minutes, spare. When preparing double tryptone soy broth, other ingredients should be doubled except distilled water.
Formula (formula):g/L
Tryptone tryptone 17.0g
Enzymatic Digest of Soybean Meal
Plant peptone 3.0g
Sodium Chloride (sodium chloride 100.0g)
Dipotassium Phosphate
Two potassium hydrogen phosphate 2.5G
Dextrose glucose 2.5G
PH7.3 + 0.2 (25 C)