BMGY/BMMY Medium Base
BMGY/BMMY Medium Base Formula
所屬分類：
Microbial culture medium
產(chǎn)品編號(hào)：
11181.
產(chǎn)品用途：
產(chǎn)品規(guī)格：
保質(zhì)期/天：
培養(yǎng)基公司報(bào)價(jià)QQ：
1772901588
微生物培養(yǎng)基公司銷售部：
400-6177-016
培養(yǎng)基生產(chǎn)廠家：
青島日水生物
關(guān)鍵詞：
客戶留言
產(chǎn)品描述
BMGY/BMMY Medium Base
11181
BMGY/BMMY Medium Base To induce the expression of Pichia pastoris, YNB(12154) and biotin were added, and 1% glycerol or 0.5% methanol was added during induction.
Preparation method: Weigh 44.1g of this product in 800mL distilled water or deionized water, heat and boil until completely dissolved, autoclave at 121℃ for 15 minutes, and cool. For aseptic operation, 100ml of filtered 13.4%YNB solution and 2ml filtered 0.02% biotin solution were added. Before induction, BMMY medium was prepared by adding 100ml 5% methanol aqueous solution filtered for sterilization. BMGY medium was prepared by adding 100ml sterile 10% glycerol solution.
BMGY/BMMY Medium Base Formula:g/L
Peptone 20.0g
Yeast Extract 10.0g
pH6.0 PBS Broth 100mmol
