無菌接觸皿培養(yǎng)基的配方和配制
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
英文名稱：Contact Plates Medium
產(chǎn)品編號(hào)：12725
產(chǎn)品規(guī)格：250g/瓶
產(chǎn)品用途：用于環(huán)境表面細(xì)菌檢測(cè)和菌落總數(shù)的測(cè)定
Formula(配方)： g/L
Pancreatic Digest of Casein （胰酪蛋白胨） 15.0g
Enzymatic Digest of Soybean Meal（大豆蛋白胨 5.0g
Sodium Chloride（氯化鈉） 5.0g
Tween 80（吐溫80） 5.0g
Lecithin（卵磷脂） 0.7g
Agar（瓊脂） 15.0g
pH7.3±0.2 （25℃）
配制方法:
稱取本品45.7g于1L蒸餾水或去離子水中（可按比例增加或減少配制量）,加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
Contact the dish culture medium
The name of the Plates is Contact Medium
Product number: 12725
Product specification: 250g/ bottle
Usage: used for detecting bacteria on environmental surface and measuring the total number of colonies
Formula: g/L
Pancreatic Digest of Casein (Pancreatic Casein) 15.0g
The Enzymatic Digest of Soybean Meal (soy peptone 5.0 g
Sodium Chloride (nacl) 5.0 g
Tween 80 (twain 80) 5.0g
Lecithin 0.7g
Agar (Agar) 15.0g
PH7.3 + / - 0.2 (25 ℃)
Preparation method:
According to take this product 45.7 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.
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