改良布氏肉湯培養(yǎng)基的配方
發(fā)布時間：
2022-12-27
作者：
日水生物
改良布氏肉湯培養(yǎng)基的配方配制方法:
稱取本品28.6g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱煮沸至完全溶解，分裝,116℃高壓滅菌20鐘,備用。
Formula(改良布氏肉湯培養(yǎng)基配方)： g/L
Tryptone （胰蛋白胨） 10.0g
Peptone(蛋白胨) 10.0g
Dextrose(葡萄糖) 1.0g
Yeast Extract(酵母浸膏) 1.0g
Sodium Chloride(氯化鈉) 5.0g
Sodium Thioglycollate
(硫乙醇酸鈉) 1.5g
Sodium metabisulfite
(偏重亞硫酸鈉) 0.1g
pH7.3±0.2（25℃）
Preparation method:
Weigh 28.6g of this product in 1L distilled water or deionized water
Medium (can increase or decrease proportionally), heat and boil
Until completely dissolved, repackaging, 116 ℃ high pressure sterilization for 20 minutes, and set aside.
Formula: g/L
Tryptone (Tryptone) 10.0g
Peptone(Peptone) 10.0g
Dextrose(glucose) 1.0g
Calcium Extract(Yeast Extract) 1.0g
Sodium Chloride (nacl) 5.0 g
Sodium Thioglycollate
(sodium thioethanolate) 1.5g
Sodium metabisulfite
(partial sodium sulfite) 0.1g
PH7.3 + / - 0.2 (25 ℃)