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腦-心浸萃液態(tài)培養(yǎng)基的配制[方法步驟]

發(fā)布時間:

2022-12-27

作者:

日水生物


腦-心浸萃液態(tài)培養(yǎng)基配制方法: 稱取本品37.0g于1L蒸餾水或去離子水中可按比例增加或減少配制量),加熱溶解,分裝,121℃高壓滅菌15分鐘,冷暗儲存?zhèn)溆茫酒飞詼啙帷?/span>
 
Formula腦-心浸萃液態(tài)培養(yǎng)基配方: g/L
 
Proteose示蛋白胨:10.0g
Sodium Chloride氯化鈉:5.0g
Disodium Hydrogen Phosphate 
磷酸氫二鈉: 2.5g
Dextrose葡萄糖:2.0g
Heart Extract牛心浸粉:9.8g
Brains Extract牛腦浸粉:7.7g
pH7.4±0.225℃
Preparation method: This product is called 37.0g in 1L distilled water or deionized water to increase or reduce the amount of preparation), heat dissolve, separate, 121 centigrade high pressure sterilization for 15 minutes, cold and dark storage reserve, this product is slightly turbid.
Formula formula):g/L
Proteose peptone: 10.0g
Sodium Chloride sodium chloride: 5.0g
Disodium Hydrogen Phosphate
Two sodium hydrogen phosphate: 2.5G
Dextrose glucose: 2.0g
Heart Extract cow heart dipping powder: 9.8g
Brains Extract bovine brain dipping powder: 7.7g
PH7.4 + 0.225 C