瓊脂培養(yǎng)基M三塘鐵瓊脂的制備方法
發(fā)布時(shí)間：
2022-12-27
作者：
山東日水培養(yǎng)基
瓊脂培養(yǎng)基M（三塘鐵瓊脂）配制方法:稱取本品65.0g于1L蒸餾水或去離子水中，加熱煮沸至完全溶解，分裝試管，121℃高壓滅菌15分鐘,滅菌結(jié)束后制成高層斜面，備用。
配方)： g/L
酪蛋白胰酶消化物：10.0g
動(dòng)物組織胃酶消化物：10.0g
乳糖：10.0g
蔗糖：10.0g
檸檬酸鐵胺：0.2g
葡萄糖：1.0g
硫代硫酸鈉：0.3g
肉浸粉：3.0g
氯化鈉： 5.0g
酵母浸粉：3.0g
酚磺酞：0.025g
瓊脂：13.0g
pH7.4±0.2(25℃)
Agar medium M (three pond iron agar) preparation method: This product is called 65.0g in 1L distilled water or deionized water, heated and boiled to completely dissolved, divided into test tubes, 121 centigrade for 15 minutes high pressure sterilization, after sterilization finished high rise slope, spare.
Formula):g/L
Casein trypsin digestion: 10.0g
Digestive enzyme digestive of animal tissue: 10.0g
Lactose: 10.0g
Sucrose: 10.0g
Ferric citrate: 0.2g
Glucose: 1.0g
Sodium thiosulfate: 0.3g
Meat dipping powder: 3.0g
Sodium chloride: 5.0g
Yeast extract: 3.0g
Phenolphthalein: 0.025g
Agar: 13.0g
PH7.4 + 0.2 (25 C)