m TEC瓊脂配方說明
發(fā)布時間：
2022-12-27
作者：
m TEC瓊脂配制方法：稱取本品45.3g于1L蒸餾水或去離子水中[可按比例增加或減少配制量],121℃高壓滅菌15分鐘,備用。
m tec agar Formula[配方]： g/L
Peptone[蛋白胨]：5.0g
Yeast Extract[酵母浸粉]：3.0g
Lactose[乳糖]：10.0g
Sodium Chloride[氯化鈉]：7.5g
Dipotassium Phosphate[ 磷酸氫二鉀]：3.3g
Monopotassium Phosphate[磷酸二氫鉀]：1.0g
Sodium Lauryl Sulfate[月桂基硫酸鈉]：0.2g
Deoxysodium Cholate[去氧膽酸鈉]：0.1g
Bromcresol Purple[溴甲酚紫]：0.08g
Bromophenol Red[溴酚紅]：0.08g
Agar[瓊脂]：15.0g
pH7.3±0.2[25℃]
METHODS: 45.3g of the product was weighed in 1L distilled water or deionized water [the amount of preparation can be increased or reduced in proportion], and sterilized at 121 C for 15 minutes.
M Tec agar Formula[formula]:g/L
Peptone[peptone]:5.0g
Yeast Extract[yeast extract powder]:3.0g
Lactose[lactose]:10.0g
Sodium Chloride[sodium chloride]:7.5g
Dipotassium Phosphate[hydrogen phosphate two potassium]:3.3g
Monopotassium Phosphate[potassium dihydrogen phosphate]:1.0g
Sodium Lauryl Sulfate[lauryl sodium sulphate]:0.2g
Deoxysodium Cholate[sodium deoxycholate]:0.1g
Bromcresol Purple[bromo cresol violet]:0.08g
Bromophenol Red[bromine phenol red]:0.08g
Agar[agar]:15.0g
PH7.3 + 0.2[25 C]