2216E瓊脂的配方

發(fā)布時(shí)間：

2022-12-27

作者：

山東培養(yǎng)基公司日水

2216E瓊脂配制方法：稱取本品55.1g于1L蒸餾水或去離子水中[可按比例增加或減少配制量],加熱煮沸至完全溶解，121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻，以防瓊脂沉積于底部而凝固，冷至50℃左右，傾注平板。

2216E Agar Formula[配方]： g/L

Peptone[蛋白胨]：5.0g

Yeast Extract[酵母浸粉]：1.0g

Ferric citrate[檸檬酸鐵]：0.1g

Sodium Chloride[氯化鈉]：19.45g

Magnesium Chloride[氯化鎂]：8.8g

Magnesium Sulfate[硫酸鎂]：3.24g

Calcium Chloride [氯化鈣]：1.8g

Potassium Chloride[氯化鉀]：0.55g

Sodium Bicarbonate[碳酸氫鈉]：0.16g

Potassium bromide [溴化鉀]：0.08g

Strontium chloride[氯化鍶]：0.034g

Boric Acid [硼酸]：0.022g

Sodium silicate[硅酸鈉]：0.004g

Sodium fluoride[氟化鈉]：0.0024g

Ammonium Nitrate[硝酸銨]：0.0016g

Disodium Hydrogen Phosphate[磷酸氫二鈉]：0.008g

Agar[瓊脂]：14.0g

pH7.6±0.2[25℃]

2216E agar preparation method: weigh 55.1g in 1L distilled water or deionized water [can increase or reduce the amount of preparation in proportion], heat and boil until completely dissolved, high pressure sterilization at 121 C for 15 minutes, shake well after sterilization, in order to prevent agar from depositing on the bottom and solidifying, cold to about 50 C, pour into the plate.

2216E Agar Formula[formula]:g/L

Peptone[peptone]:5.0g

Yeast Extract[yeast extract powder]:1.0g

Ferric citrate[citric acid iron]:0.1g

Sodium Chloride[NaCl]:19.45g

Magnesium Chloride[]:8.8g magnesium chloride

Magnesium Sulfate[Magnesium Sulfate]:3.24g

Calcium Chloride [CaCl2]:1.8g

Potassium Chloride[potassium chloride]:0.55g

Sodium Bicarbonate[sodium bicarbonate]:0.16g

Potassium bromide [potassium bromide]:0.08g

Strontium chloride[strontium chloride]:0.034g